pH-sensitive vibrational probe reveals a cytoplasmic protonated cluster in bacteriorhodopsin
نویسندگان
چکیده
منابع مشابه
Structures and spectral signatures of protonated water networks in bacteriorhodopsin.
Networks of internal water molecules are thought to provide proton transfer pathways in many enzymatic and photosynthetic reactions. Extremely broad absorption continua observed in recent IR spectroscopic measurements on the photodriven proton pump bacteriorhodopsin (BR) suggest such networks may also serve as proton storage and release sites for these reactions. By combining electronic structu...
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In a recent publication, the structure, dynamics, and rovibrational spectrum of the hydrated proton in the “magic” 21-water cluster H+ H2O 21 was studied. It was found that dynamical effects were critical in determining the vibrational properties of such clusters. This result was gathered from an analysis of the vibrational density of states including a the Fourier transform of the nuclear velo...
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We demonstrated that the liposomes composed of dioleolylphosphatidylethanolamine/cholesterol/oleic acid (4:4:2) dramatically release their contents at a pH of less than or equal to 6.0 and are capable of delivering their contents into the cytoplasm of higher plant protoplasts. This is shown by using a soluble fluorescent dye, calcein, as a liposome-entrapped marker. We found that calcein fluore...
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Due to the lack of a proper imaging approach, a veracious pH map of normal and abnormal cell is still rare. In this work, we presented a rhodamine-salicylaldehyde combination (Rh-SA2) as a novel pH probe, which has dual sensitive units for both acidic and basic environment. This dual sensitive probe acts like a chameleon in living cells and offers the doubling guarantees for endocellular pH map...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2017
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1707993114